علوم زیستی دریا
Somayeh Zangeneh; Solmaz Kakeshian; Solmaz Shirali; Ahmad Savari; Hossein Najafzadeh Varzi; Abdolali Movahedinia; Mohammad Zakeri
Abstract
The present study has performed to investigate the effects of different levels of soy and garlic phytoestrogens on ovarian structure in Yellowfin seabream (Acanthopagrus latus). A total of 105 Yellowfin seabream were divided in seven groups including a control group that were received standard ...
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The present study has performed to investigate the effects of different levels of soy and garlic phytoestrogens on ovarian structure in Yellowfin seabream (Acanthopagrus latus). A total of 105 Yellowfin seabream were divided in seven groups including a control group that were received standard food without any extract and six treatment groups that were received different doses of soy (2/5, 5 and 7/5%) and garlic (0/5, 1 and 2%) hydroalcoholic extract. Fish were fed daily at a rate of 3% of body weight twice for 14 days. Sampling was done in days of 0, 7, 10 and 14. In every time, a section of mid portion of gonad was cut for histological examination. The results showed that different doses of soy and garlic phytoestrogens have various effects on growth and development of gonad in Yellowfin seabream as a protandrous hermaphrodite fish, so that increase in dose and time of consumption of food containing garlic hydroalchoholic extract cause increase in number of vitellogenesis follicles and finally growth and development of ovary, while consumption of food containing soy hydroalcoholic extract cause observing of testis and immature ovaries containing previtellogenesis follicle and prevents gonad development from testis to ovary in protandrous hermaphrodite fish.
علوم زیستی دریا
h p; r a; a m
Abstract
To determine the cellular and adaptations changes in mitochondria -rich cells under different environmental salinities, Sparidentex hasta as a urihaline has been studied.180 fish weighing 150 ± 0.5 gr and 23±1 cm length, were directly exposure to (5 , 20 , 40 and 60ppt) salinitiesfor a ...
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To determine the cellular and adaptations changes in mitochondria -rich cells under different environmental salinities, Sparidentex hasta as a urihaline has been studied.180 fish weighing 150 ± 0.5 gr and 23±1 cm length, were directly exposure to (5 , 20 , 40 and 60ppt) salinitiesfor a week.Sampling was performed on days 1,2 and 7 and three fish from each tank ( 12 fish from each treatment ) were cached at each time. After preparing, samples fixed in glutaraldehyde 2/5 % solution and washed with 0.1M (pH=7.4) buffer phosphate. Samples were dehydrated in increasing ethanol series ( from 50 % to pure ethanol )followed by 100 % acetone.Tissue blocks were frozen by liquid nitrogen and then immediately followed by scanning electron microscope LEO ( Model 1455VP) with the voltage 15Kv. The upper levels of mitochondria -rich cells under scanning electron microscope revealed according to the apical openings, three types of mitochondria -rich cells derived apertures, shallow and deep recognizable.The number and size of mitochondria -rich cells in the apical openings in 60ppt salinity increase on the first day compared to rest of the treatments .During the experiment, the density and size of the openings in the mitochondria -rich cells in the lower salinity of the sea water was increased but in higher salinity of sea watersize and density of openingsincreased during experimental time.